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2008 and in press
Barbante A, Irons S, Hawes C,
Frigerio L, Vitale A, Pedrazzini E. Anchorage to the cytosolic face of the ER
membrane: a new strategy to stabilize a cytosolic recombinant antigen in
plants. Plant Biotechnol J (in press).
de Virgilio M, De Marchis F,
Bellucci M, Mainieri D, Rossi M, Benvenuto E, Arcioni S, Vitale A. The human
immunodeficiency virus antigen Nef forms protein bodies in leaves of transgenic
tobacco when fused to zeolin (in press)
Delannoy M, Alves G, Vertommen D, Ma
JKC, Boutry M, Navarre C (2008) Identification of peptidases in the Nicotiana tabacum leaf intercellular
fluid. Proteomics (in press).
Floß D, Sack M, Rademacher T,
Fischer R, Conrad U (2008) Biochemical and functional characterization of
anti-HIV antibody ELP fusion proteins from transgenic plants. Plant
Biotechnology Journal (in press).
Irons SL, Nuttall J,
Floß DM, Frigerio L, Kotzer AM,
Hawes C (2008) Fluorescent protein fusions to a human immunodeficiency virus
monoclonal antibody reveal its intracellular transport through the plant
endomembrane system. Plant Biotechnol J (in press)
Jin C, Altmann F, Strasser R, Mach
L, Schähs M, Kunert R, Rademacher T, Glössl J, Steinkellner H (2008)
A plant derived human monoclonal antibody induces an anti-carbohydrate immune
response in rabbits. Glycobiology 18, 235-241.
Jin C, Hantusch G, Hemmer W,
Stadlmann J, Altmann F (2008) Affinity of IgE and IgG against cross-reactive carbohydrate
determinants on plant and insect glycoproteins. J. Allergy Clin. Immunol. 121,
185-190.
Langhans M, Marcote MJ, Pimpl P,
Virgili-Lopez G, Robinson DG, Aniento F (2008) in vivo trafficking and
localization of p24 proteins in plant cells. Traffic (in press).
Platis D, Labrou NE (2008) Affinity
chromatography for the purification of therapeutic proteins from transgenic
maize using immobilized histamine. J Sep Sci (in press).
Rademacher T, Sack M, Arcalis E,
Stadlmann J, Balzer S, Altmann F, Quendler H, Stiegler G, Kunert R, Fischer R,
and Stoger E (2008) Recombinant antibody 2G12 produced in maize endosperm
efficiently neutralizes HIV-1 and contains predominantly single-GlcNAc
N-glycans. Plant Biotechnology J 6, 189-201.
Ramessar K, Rademacher T, Sack M,
Stadlmann J, Platis D, Stiegler G, Labrou N, Altmann F, Ma JKC, Stöger E,
Capell T, Christou P (2008) Cost-effective production of a vaginal protein
microbicide to prevent HIV transmission. Proc Natl Acad Sci (in press).
Ramessar K, Sabalza M, Capell T,
Christou P (2008) Maize plants: an ideal production platform for effective and
safe molecular pharming. Plant Science (in press).
Ramessar K, Capell T, Christou P
(2008) Molecular pharming in cereal crops. Phytochem Rev (in press).
Sainsbury F, Lavoie P-O,
D’Aoust M-A, Vezina L-P, Lomonossoff GP (2008) Expression of multiple
proteins using full-length and deleted versions of Cowpea mosaic virus RNA-2.
Plant Biotechnol J 6, 82-92.
Sparrow PAC (2008) GM risk
assessment: Transgenic wheat, barley and oats: production and characterization.
In: Faye L, Gomord V (eds) Recombinant Pharmaceutical Proteins from Plants.
Methods in Biotechnology, Humana Press (in press).
Sparrow PAC, Twyman RM (2008)
Biosafety and Risk Assessment of Plant Made Pharmaceuticals. In: Faye L, Gomord V (eds) Recombinant
Pharmaceutical Proteins from Plants. Methods in Biotechnology, Humana Press (in
press).
Spök A, Twyman RM, Fischer R,
Ma JKC, Sparrow PAC (2008) Evolution of a regulatory framework for plant-made
pharmaceuticals. Trends Biotechnol (in press).
Strasser R, Stadlmann J, Schähs
M, Stiegler G, Quendler H, Mach L, Glössl J, Weterings K, Pabst M,
Steinkellner H (2008) Generation of glyco-engineered Nicotiana benthamiana for
the production of monoclonal antibodies with a homogeneous human-like N-glycan
structure. Plant Biotechnol J 6, 392-402.
Van Droogenbroeck B, De Wilde K,
Depicker A (2008) Production of antibody fragments in Arabidopsis seeds. In:
Faye L, Gomord V (eds) Recombinant Pharmaceutical Proteins from Plants. Methods
in Biotechnology, Humana Press (in press).
2007
Bock R (2007) Plastid biotechnology:
prospects for herbicide and insect resistance, metabolic engineering and
molecular farming. Curr Opin Biotechnol 18, 100-106.
Capuano F, Beaudoin F, Napier JA,
Shewry PR. (2007) Properties and exploitation of oleosins. Biotechnol Adv. 25,
203-206.
Maggio C, Barbante A, Ferro F,
Frigerio L, Pedrazzini E (2007) Intracellular sorting of the tail-anchored
protein cytochrome b5 in plants: a comparative study using different isoforms
from rabbit and Arabidopsis. J Exp Bot. 58, 1365-1379.
Marusic C, Nuttall J, Buriani G,
Lico C, Lombardi R, Baschieri S, Benvenuto E and Frigerio L (2007) Expression,
intracellular targeting and purification of HIV Nef variants in tobacco cells.
BMC Biotechnol 26, 7-12.
Pabst M, Bondili JS, Stadlmann J,
Mach L, Altmann F (2007) Mass + retention time = structure: A strategy for the
analysis of N-glycans by carbon LC-ESI-MS and its application to fibrin
N-glycans. Anal Chem 79, 5051-5057.
Ramessar K, Peremarti A, Gomez
Galera S, Naqvi S, Moralejo M, Muñoz M, Capell T and Christou P (2007)
Biosafety and risk assessment framework for selectable marker genes in
transgenic crop plants. A case of the science not supporting the politics. Transgenic
Res 16, 261-280.
Sack M, Paetz A, Kunert R, Bomble M,
Hesse F, Stiegler G, Fischer R, Katinger H, Stoeger E, Rademacher T (2007)
Analysis of the broadly neutralizing human anti-HIV-1 antibody 2F5 produced in
transgenic tobacco BY2 suspension cells. FASEB J 21, 1655-1664.
Sainsbury F, Cañizares MC,
Lomonossoff GP (2007) Cowpea mosaic virus-based expression vectors. In: Virus
expression vectors 2007 (Hefferon KL, ed) Transworld Research Network, pp
339-355.
Schähs M, Strasser R, Stadlmann
J, Kunert R, Rademacher T, Steinkellner H (2007) Production of monoclonal
antibodies in plants with a humanized glycosylation pattern. Plant Biotechnol
J. 5, 657-663.
Snowden, CJ, Leborgne-Castel N,
Wootton LJ, Hadlington JL, Denecke J (2007) In vivo analysis of the lumenal
binding protein (BiP) reveals multiple functions of its ATPase domain. Plant J
52, 987-1000.
Sparrow PAC, Irwin JA, Dale PJ,
Twyman RM, Ma JKC (2007) Pharma-Planta: road testing the developing regulatory
guidelines for plant-made pharmaceuticals. Transgenic Res 16, 147-161.
Strasser R, Bondili JS, Schoberer J,
Svoboda B, Liebminger E, Glossl J, Altmann F, Steinkellner H, Mach L (2007)
Enzymatic properties and subcellular localization of Arabidopsis b-N-acetylhexosaminidases.
Plant Physiol 145, 5-16.
Strasser R, Bondili JS, Vavra U,
Schoberer J, Svoboda B, Glössl J, Léonard R, Stadlmann J, Altmann F, Steinkellner H, Mach L (2007) A unique β1,3-galactosyltransferase
is indispensable for the biosynthesis of complex N-glycans in plants. Plant
Cell 19, 2278-2292.
Twyman RM, Schillberg S, Fischer R
(2007) Molecular farming of antibodies in plants. In: Ranalli P (ed)
Improvements of Crop Plants for Industrial End Uses. Springer-Verlag, NY pp
435-469.
Van Droogenbroeck B, Cao J,
Stadlmann J, Altmann F, Colanesi S, Hillmer S,. Robinson D G, van Lerberge E,
Terryn N, van Monatgu M, Liang M, Depicker A, de Jaeger G (2007) Aberrant
localization and underglycosylation of highly accumulating single chain Fv-Fc
antibodies in transgenic Arabidopsis seeds. Proc Natl Acad Sci USA 104,
1430-1435
2006
Alamillo, J.M., Monger, W., Sola,
I., Garcia, B., Perrin, Y., Bestagno, M., Burrone, O.R., Sabella, P.,
Plana-Duran, J., Enjuanes, L., Lomonossoff, G.P. and Garcia, J.A. (2006). Use
of virus vectors for the expression in plants of active full-length and single
chain anti-coronavirus antibodies. Biotechnol J 1, 1103-1111.
Avesani L (2006) I farmaci dalle piante, il futuro
è già arrivato. .L’informatore Agrario 31, 73-74.
Cañizares, M.C., Liu, L., Perrin,
Y., Tsakiris, E. and Lomonossoff, G.P. (2006) A bipartite system for the
constitutive and inducible expression of high levels of foreign proteins in
plants. Plant Biotechnol J 4, 183-193.
Chakauya E, Chikwamba R, Rybicki EP
(2006). Riding the tide of biopharming in Africa: considerations for risk
Assessment. South African Journal of Science 102:284
Foresti, O., Dasilva, L.L., and
Denecke, J. (2006). Overexpression of the Arabidopsis Syntaxin PEP12/SYP21
Inhibits Transport from the Prevacuolar Compartment to the Lytic Vacuole in
Vivo. Plant Cell 18, 2275-2293.
Klein EM, Mascheroni L, Pompa A,
Ragni L, Weimar T, Lilley KS, Dupree P, Vitale A (2006). Plant endoplasmin
supports the protein secretory pathway and has a role in proliferating tissues.
Plant J. 48, 657-673.
Liu, L. and Lomonossoff, G.P. (2006)
A site-directed mutagenesis method utilising large double-stranded DNA
templates for the simultaneous introduction of multiple changes and sequential
multiple rounds of mutation: Application to the study of whole viral genomes.
J. Virological Methods, 137, 63-71.
Navarre C, Delannoy M, Lefebvre B,
Nader J, Vanham D, Boutry M (2006) Expression and secretion of recombinant
outer-surface protein A from the Lyme disease agent, Borrelia burgdorferi, in
Nicotiana tabacum suspension cells. Transgenic Res 15:325-335.
Nicholson, L., Cañizares,
M.C. and Lomonossoff, G.P. (2006) Production of vaccines in GM Plants. In:
Plant Biotechnology: Current and Future Uses of Genetically Modified Crops,
(Halford, N., ed.), Wiley, pp164-192
Obregon P, Chargelegue D, Drake PMW,
Prada A, Nuttall J, Frigerio L, Ma JKC (2006) HIV-1 p24-immunoglobulin fusion
molecule: A new strategy for plant-based protein production. Plant Biotechnol J
4: 195-207.
Twyman RM, Schillberg S, Fischer R
(2006) Production of therapeutic antibodies and heterologous recombinant
proteins in plant systems. In: Kayser O, Quax W (eds) Medicinal Plant
Biotechnology. Wiley-VCH, Weinheim, Germany pp 319-344.
Yusibov V, Rabindran S, Commandeur
U, Twyman RM, Fischer R (2006) The potential of plant virus vectors for vaccine
production. Drugs in R&D 7, 203-217.
2005
Abranches R, Marcel S, Arcalis E,
Altmann F, Fevereiro P, Stoger E (2005) Plants as bioreactors: a comparative
study suggests that Medicago truncatula is a promising production system. J
Biotechnol 120:121-134
Altpeter F, Baisakh N, Beachy R,
Bock R, Capell T, Christou P, Daniell H, Datta K, Datta S, Dix PJ, Fauquet C,
Huang N, Kohli A, Mooibroek H, Nicholson L, Nguyen TT, Nugent G, Raemakers K,
Romano A, Somers DA, Stoger E, Taylor N, Visser R (2005) Particle bombardment
and the genetic enhancement of crops: myths and realities. Mol Breeding
15:305-327.
Bellucci M, De Marchis F, Mannucci
R, Bock R, Arcioni S (2005). Cytoplasm and chloroplast are not suitable
sub-cellular localizations for β-zein accumulation in transgenic plants. J
Exp Bot 56:1205-1212.
Canizares MC, Lomonossoff GP,
Nicholson L (2005) Development of cowpea mosaic virus-based vectors for the
production of vaccines in plants. Expert Rev Vaccines 4:687-967.
Canizares MC, Nicholson L,
Lomonossoff GP (2005) Use of viral vectors for vaccine production in plants.
Immunol Cell Biol 83:263-270.
DaSilva LL, Taylor JP, Hadlington
JL, Hanton SL, Snowden CJ, Fox SJ, Foresti O, Brandizzi F, Denecke J (2005)
Receptor salvage from the prevacuolar compartment is essential for efficient
vacuolar protein targeting. Plant Cell 17:132-148.
Liu L, Cañizares MC, Monger
W, Perrin Y, Tsakiris E, Porta C, Shariat N, Nicholson L, Lomonossoff G P
(2005) Cowpea mosaic virus-based systems for the production of antigens and
antibodies in plants. Vaccine 23:1788-1792.
Ma JK, Barros E, Bock R, Christou P,
Dale PJ, Dix PJ, Fischer R, Irwin J, Mahoney R, Pezzotti M, Schillberg S,
Sparrow P, Stoger E, Twyman RM (2005) Molecular farming for new drugs and
vaccines. Current perspectives on the production of pharmaceuticals in
transgenic plants. EMBO Rep 6:593-599.
Ma JK, Chikwamba R, Sparrow P,
Fischer R, Mahoney R, Twyman RM (2005) Plant-derived pharmaceuticals - the road
forward. Trends Plant Sci 10:580-585.
Nicholson L, Gonzalez-Melendi P, van
Dolleweerd C, Tuck H, Ma J KC, Fischer R, Christou P, Stoger E (2005) A
recombinant multimeric immunoglobulin expressed in rice shows
assembly-dependent subcellular localization in endosperm cells. Plant
Biotechnol J 3:115-27.
Nuttall J, Ma JKC, Frigerio L (2005)
A functional antibody lacking N-linked glycans is efficiently folded, assembled
and secreted by tobacco mesophyll protoplasts. Plant Biotechnol J 3:497-504.
Schiermeyer A, Schinkel H, Apel S
Fischer R Schillberg S (2005). Production of Desmodus rotundus plasminogen
activator 1
(DSPA1)
in tobacco is hampered by proteolysis. Biotechnol Bioeng 89:848-58.
Schillberg S, Twyman RM, Fischer R
(2005) Opportunities for recombinant antigen and antibody expression in
transgenic plants - technology assessment. Vaccine 23:1764-9.
Schinkel H, Schiermeyer A, Soeur R,
Fischer R, Schillberg S (2005) Production of an active recombinant
thrombomodulin derivative in transgenic tobacco plants and suspension cells.
Transgenic Res 14: 251-259.
Sorrentino A, Schillberg S, Fischer
R, Rao R, Porta R, Mariniello L (2005) Recombinant human tissue
transglutaminase produced into tobacco suspension cell cultures is active and
recognizes autoantibodies in the serum of coeliac patients. Int J Biochem Cell
Biol 37: 842-52.
Stoger E, Ma JKC, Fischer R,
Christou P (2005) Sowing the seeds of success: pharmaceutical proteins from
plants. Curr Opin Biotechnol 16:167-173.
Stoger E, Sack M, Nicholson L,
Fischer R, Christou P (2005) Recent progress in plantibody technology. J Curr
Pharmaceutical Design 11:2439-2457.
Twyman RM, Schillberg S Fischer R
(2005) The transgenic plant market in the pharmaceutical industry. Expert Opin
Emerging Drugs 10:185-218.
Uhde K, Fischer R, Commandeur U
(2005) Expression of multiple foreign epitopes presented as synthetic antigens
on the surface of potato virus X particles. Arch Virol 150:327-340.
Vitale A, Pedrazzini E (2005)
Recombinant pharmaceuticals from plants: the plant endomembrane system as
bioreactor. Mol Interv 5:216-225.
2004
Arcalis E, Marcel S, Altmann F,
Kolarich D, Drakakaki G, Fischer R, Christou P, Stoger E (2004) Unexpected
deposition patterns of recombinant proteins in post-ER compartments of wheat
endosperm. Plant Physiol 136:3457-66.
Bock R, Khan MS (2004) Taming
plastids for a green future. Trends Biotechnol 22:311-318.
Butaye KM, Goderis IJ, Wouters PF,
Pues JM, Delaure SL, Broekaert WF, Depicker A, Cammue BP, De Bolle MF (2004)
Stable high-level transgene expression in Arabidopsis thaliana using gene
silencing mutants and matrix attachment regions. Plant J 39:440-449.
De Buck S, Windels P, De Loose M,
Depicker A (2004) Single copy T-DNAs integrated at different positions in the
Arabidopsis genome display uniform and comparible β-glucuronidase
accumulation levels. Cell Mol Life Sci 61: 2632-45.
Fischer R, Schillberg S (eds) (2004)
Molecular Farming: Plant-made Pharmaceuticals and Technical Proteins .Wiley-VCH
Verlag GmbH & Co. KGaA, Weinheim, Germany.
Fischer R, Stoger E, Schillberg S,
Christou P, Twyman RM (2004) Plant based production of biopharmaceuticals. Curr
Opin Plant Biol 7:152-8.
Garcia-Perez RD, van Houdt H,
Depicker A (2004) Spreading of post-transcriptional gene silencing along the
target gene promotes systemic silencing. Plant J 38:594-602.
Hellwig S, Drossard J, Twyman RM,
Fischer R (2004) Plant cell cultures for the production of recombinant
proteins. Nature Biotechnol 22:1415-22.
Sanders M, Lannoo N, Maddelein W,
Depicker A, Van Montagu M, Cornelissen M, Jacobs J (2004) The preferred route
for the degradation of silencing target RNAs in transgenic plants depends on
pre-established silencing conditions. Nucleic Acids Res 32:3400-3409.
Sriraman R, Bardor M, Sack M,
Vaquero C, Faye L, Fischer R, Finnern R, Lerouge P (2004) Recombinant anti-hCG
antibodies retained in the endoplasmic reticulum of transformed plants lack
core-xylose and core-α(1,3)-fucose residues. Plant Biotechnol J 2:279-87.